咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人正常細(xì)胞>HTB-103KATO III細(xì)胞, 人胃癌細(xì)胞

KATO III細(xì)胞, 人胃癌細(xì)胞

簡(jiǎn)要描述:KATO III細(xì)胞, 人胃癌細(xì)胞
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞;細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號(hào):HTB-103
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2025-06-28
  • 訪  問(wèn)  量:2159

產(chǎn)品分類

Product Category

詳細(xì)介紹

KATO III細(xì)胞, 人胃癌細(xì)胞

OrganismHomo sapiens, human
Tissue

stomach:derived from metastatic pleural effusion; supraclavicular and axillary lymph nodes and Douglas cul-de-sac

Product Formatfrozen
Morphologyspherical
Culture Propertiesmixed, adherent and suspension
Biosafety Level1
Diseasegastric carcinoma
Age55 years adult
Gendermale
EthnicityAsian
Storage Conditionsliquid nitrogen vapor phase
KaryotypeThe stemline chromosome number is hypotetraploid with the 2S component occurring at 6.2%. Nine markers were common to most S metaphases, four markers were less frequent. One (occasionally 2 copies) homogenous staining region (HSR) (t(11;HSR) was present in all metaphases examined, but no double minutes (DM) were detected.
Clinical Data

55 years adult

Asian

male

TumorigenicYes
Effects

Yes, in cheek pouches of anti thymocyte serum treated hamsters

No, in nude mice

Complete Growth MediumThe base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
KATO III細(xì)胞, 人胃癌細(xì)胞
Subculturing

Protocol: Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. 1. Remove culture medium with floating cells to a centrifuge tube. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum, which contains trypsin inhibitor. 2.. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 10 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. 3. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. 4. To remove trypsin-EDTA solution, transfer cell suspension to the centrifuge tube with the medium and cells from step #1 and spin at approximay 125 xg for 5 to10 minutes. 5. Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels. 6. Place culture vessels in incubator at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:8 is recommended

Medium Renewal: Every 2 to 3 days

Cryopreservation

Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Atmosphere: 5% CO2 in air recommended

Temperature: 37°C          






















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
主站蜘蛛池模板: 中文字幕91在线| 亚洲aⅴ男人的天堂在线观看| 一本之道在线视频| 欧美中文字幕在线播放| 人人妻人人狠人人爽| 精品无码久久久久久久久久| 国产精品亚洲精品日韩已满| acg全彩无遮挡口工漫画网址| 日韩一区二区在线免费观看| 人间**电影8858| 里番全彩本子库acg污妖王| 妲己高h荡肉呻吟np| 九九影院理论片在线观看一级| 白嫩光屁股bbbbbbbbb| 国产在线不卡一区二区三区| a级黄色片视频| 小仙女app2021版最新| 久久精品aⅴ无码中文字字幕重口| 玉蒲团之天下第一| 国产亚洲欧美日韩俺去了| 456在线视频| 岛国大片免费观看| 久久综合九色综合欧美就去吻| 王爷晚上含奶h嗯额嗯| 办公室开档情趣内衣做爽视频| 老师在办公室被躁在线观看| 国产精品亚洲片在线观看不卡| 91香蕉视频黄色| 性满足久久久久久久久| 久久综合色之久久综合| 欧美一级高清免费播放| 亚洲国产精品区| 男女一级做片a性视频| 国产乡下三级全黄三级| 57pao国产成永久免费视频| 成人免费小视频| 中文字幕第13亚洲另类| 欧美乱妇高清无乱码在线观看| 亚洲无线一二三四区| 福利在线小视频| 国产主播精品福利19禁vip|